Elisakit.com
Call to Order:  1 300 ELISAK (1 300 354 725)  Contact Us

Call to Order:  

1 300 ELISAK (1 300 354 725)

  Contact Us

Quality

  • Elisakit.com sources and tests matched capture and detection antibody pairs to ensure high sensitivity of detection for each antigen with negligible cross-reactivity to other proteins.
  • We compare our products against kits from leading suppliers including R&D systems, Invitrogen and others. Researchers can be confident that elisakit.com kits will match or exceed the performance of competitive products.
  • Elisakit.com manufactures and stocks its kits locally in Melbourne, eliminating risks of damage to kits through exposure to heat or other adverse conditions during international shipping.

ELISAkit.com vs R&D Systems quality comparison
Hu TNF-α ELISA kits

Summary

A comparison of the assay characteristics between the elisakit.com product and a market leading manufacturer (R & D systems) was performed.   
The assays were set up to give comparative information as to the standard curves, CVs, intra-assay variability, spike testing and sensitivity.
All assays were performed using serum as the matrix.

The ELISAkit.com product easily matched the performance obtained from the market leading product across all criteria tested.

Data

Fig 1) Comparative data on standard curve

Tabulated assay data – ELISAkit.com

  Standard (pg/ml)
1000 500 250 125 62.5 31.2 15.6 7.81 0
OD 2.52 1.33 0.81 0.50 0.32 0.23 0.19 0.18 0.081
2.43 1.30 0.74 0.48 0.31 0.21 0.17 0.16 0.078
Mean 2.48 1.32 0.77 0.49 0.31 0.22 0.18 0.17 0.080
SD 0.06 0.02 0.05 0.02 0.00 0.01 0.01 0.01 0.002
CV (%) 2.54 1.40 5.85 3.19 1.35 4.79 6.62 6.66 2.668

Tabulated assay data – R&D systems kit

Standard (pg/ml)
1000 500 250 125 62.5 31.2 15.6 7.81 0
OD 1.98 1.03 0.58 0.35 0.23 0.15 0.12 0.09 0.053
1.87 1.00 0.54 0.33 0.21 0.15 0.11 0.09 0.055
Mean 1.93 1.01 0.56 0.34 0.22 0.15 0.11 0.09 0.054
SD 0.08 0.02 0.03 0.02 0.01 0.00 0.01 0.00 0.001
CV (%) 4.03 2.23 4.78 4.58 6.55 1.91 5.71 3.86 2.619

Intra-assay precision
In this assay serum was spiked with known concentrations of TNF-α and tested 4 times on one plate.

  Elisakit.com R&D Systems
Sample mean (pg/ml) 297 127.3 59 297 127.3 59
n 4 4 4 4 4 4
SD 5.88 6.02 1.66 3.54 5.89 0.79
CV (%) 1.48 3.10 2.05 1.03 3.29 0.78

Same data transposed

  Sample mean (pg/ml) n SD CV (%)
Elisakit.com 297 4 5.88 1.48
127.3 4 6.02 3.10
59 4 1.66 2.05
R&D Systems 297 4 3.54 1.03
127.3 4 5.89 3.29
59 4 0.79 0.78

Spike testing comparisonIn this assay serum was spiked with known concentrations of TNF-α (n=4).

Spike (pg/ml)
333.3 142.8 66.6
ELISAkit.com 366.9 162.3 79.6
R&D Systems 356.3 162.4 83.8

Same data with % error from expected result

Spike (pg/ml)
333.3 142.8 66.6
ELISAkit.com 366.9 (+10%) 162.3 (+14%) 79.6 (+20%)
R&D Systems 356.3 (+7%) 162.4 (14%) 83.8 (26%)

Lower limit of quantitation (LLQ)

  LLQ (pg/ml)*
Elisakit.com 37
R&D Systems 32

*The LLQ was calculated by adding 3 standard deviations to the mean of the zero standard replicates.